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fluorescent nuclear stain reddot2  (Biotium)


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    Biotium fluorescent nuclear stain reddot2
    ( A ) Tiled confocal images of a representative coronal hemisphere stained for Iba1. ( B ) Compared to saline controls (n = 5–9 per group), decreased densities of Iba1+ cells were observed in the PFC of male and female animals self-administering METH (n = 5–7 per group). * p < 0.05 vs. saline. ( C ) Left , representative tissue section showing outlines of nuclei (red) stained by the far-red nuclear stain <t>RedDot2,</t> and TUNEL staining (green). Arrows highlight TUNEL+ nuclei. Middle and right , immunostaining of the PFC and DStr for the apoptosis marker cleaved caspase 3 (CC3, green) and microglial marker Iba1 (red), counterstained with nuclear marker RedDot2 (blue). Arrows denote co-localization of all three signals. ( D ) Quantification of TUNEL+ cells in the PFC and DStr of male and female rats self-administering METH (n = 5–7 per group), MDPV (n = 6–8 per group), or saline (n = 4–9 per group). * p < 0.05 vs. corresponding saline groups. ( E ) Quantification of co-localized CC3, Iba1, and nuclei in the PFC and DStr of male and female rats self-administering METH (n = 4–6 per group), MDPV (n = 4–6 per group), or saline (n = 5–6 per group). * p < 0.05 vs. corresponding saline groups. All data are shown as mean ± SEM. Open circles represent data values from individual animals.
    Fluorescent Nuclear Stain Reddot2, supplied by Biotium, used in various techniques. Bioz Stars score: 95/100, based on 163 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescent+nuclear+stain+reddot2/pmc11118022-118-11-15?v=Biotium
    Average 95 stars, based on 163 article reviews
    fluorescent nuclear stain reddot2 - by Bioz Stars, 2026-08
    95/100 stars

    Images

    1) Product Images from "Methamphetamine and the Synthetic Cathinone 3,4-Methylenedioxypyrovalerone (MDPV) Produce Persistent Effects on Prefrontal and Striatal Microglial Morphology and Neuroimmune Signaling Following Repeated Binge-like Intake in Male and Female Rats"

    Article Title: Methamphetamine and the Synthetic Cathinone 3,4-Methylenedioxypyrovalerone (MDPV) Produce Persistent Effects on Prefrontal and Striatal Microglial Morphology and Neuroimmune Signaling Following Repeated Binge-like Intake in Male and Female Rats

    Journal: Brain Sciences

    doi: 10.3390/brainsci14050435

    ( A ) Tiled confocal images of a representative coronal hemisphere stained for Iba1. ( B ) Compared to saline controls (n = 5–9 per group), decreased densities of Iba1+ cells were observed in the PFC of male and female animals self-administering METH (n = 5–7 per group). * p < 0.05 vs. saline. ( C ) Left , representative tissue section showing outlines of nuclei (red) stained by the far-red nuclear stain RedDot2, and TUNEL staining (green). Arrows highlight TUNEL+ nuclei. Middle and right , immunostaining of the PFC and DStr for the apoptosis marker cleaved caspase 3 (CC3, green) and microglial marker Iba1 (red), counterstained with nuclear marker RedDot2 (blue). Arrows denote co-localization of all three signals. ( D ) Quantification of TUNEL+ cells in the PFC and DStr of male and female rats self-administering METH (n = 5–7 per group), MDPV (n = 6–8 per group), or saline (n = 4–9 per group). * p < 0.05 vs. corresponding saline groups. ( E ) Quantification of co-localized CC3, Iba1, and nuclei in the PFC and DStr of male and female rats self-administering METH (n = 4–6 per group), MDPV (n = 4–6 per group), or saline (n = 5–6 per group). * p < 0.05 vs. corresponding saline groups. All data are shown as mean ± SEM. Open circles represent data values from individual animals.
    Figure Legend Snippet: ( A ) Tiled confocal images of a representative coronal hemisphere stained for Iba1. ( B ) Compared to saline controls (n = 5–9 per group), decreased densities of Iba1+ cells were observed in the PFC of male and female animals self-administering METH (n = 5–7 per group). * p < 0.05 vs. saline. ( C ) Left , representative tissue section showing outlines of nuclei (red) stained by the far-red nuclear stain RedDot2, and TUNEL staining (green). Arrows highlight TUNEL+ nuclei. Middle and right , immunostaining of the PFC and DStr for the apoptosis marker cleaved caspase 3 (CC3, green) and microglial marker Iba1 (red), counterstained with nuclear marker RedDot2 (blue). Arrows denote co-localization of all three signals. ( D ) Quantification of TUNEL+ cells in the PFC and DStr of male and female rats self-administering METH (n = 5–7 per group), MDPV (n = 6–8 per group), or saline (n = 4–9 per group). * p < 0.05 vs. corresponding saline groups. ( E ) Quantification of co-localized CC3, Iba1, and nuclei in the PFC and DStr of male and female rats self-administering METH (n = 4–6 per group), MDPV (n = 4–6 per group), or saline (n = 5–6 per group). * p < 0.05 vs. corresponding saline groups. All data are shown as mean ± SEM. Open circles represent data values from individual animals.

    Techniques Used: Staining, Saline, TUNEL Assay, Immunostaining, Marker



    Similar Products

    fluorescent nuclear stain reddot2  (Biotium)
    95
    Biotium fluorescent nuclear stain reddot2
    ( A ) Tiled confocal images of a representative coronal hemisphere stained for Iba1. ( B ) Compared to saline controls (n = 5–9 per group), decreased densities of Iba1+ cells were observed in the PFC of male and female animals self-administering METH (n = 5–7 per group). * p < 0.05 vs. saline. ( C ) Left , representative tissue section showing outlines of nuclei (red) stained by the far-red nuclear stain <t>RedDot2,</t> and TUNEL staining (green). Arrows highlight TUNEL+ nuclei. Middle and right , immunostaining of the PFC and DStr for the apoptosis marker cleaved caspase 3 (CC3, green) and microglial marker Iba1 (red), counterstained with nuclear marker RedDot2 (blue). Arrows denote co-localization of all three signals. ( D ) Quantification of TUNEL+ cells in the PFC and DStr of male and female rats self-administering METH (n = 5–7 per group), MDPV (n = 6–8 per group), or saline (n = 4–9 per group). * p < 0.05 vs. corresponding saline groups. ( E ) Quantification of co-localized CC3, Iba1, and nuclei in the PFC and DStr of male and female rats self-administering METH (n = 4–6 per group), MDPV (n = 4–6 per group), or saline (n = 5–6 per group). * p < 0.05 vs. corresponding saline groups. All data are shown as mean ± SEM. Open circles represent data values from individual animals.
    Fluorescent Nuclear Stain Reddot2, supplied by Biotium, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescent+nuclear+stain+reddot2/pmc11118022-118-11-15?v=Biotium
    Average 95 stars, based on 1 article reviews
    fluorescent nuclear stain reddot2 - by Bioz Stars, 2026-08
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Tiled confocal images of a representative coronal hemisphere stained for Iba1. ( B ) Compared to saline controls (n = 5–9 per group), decreased densities of Iba1+ cells were observed in the PFC of male and female animals self-administering METH (n = 5–7 per group). * p < 0.05 vs. saline. ( C ) Left , representative tissue section showing outlines of nuclei (red) stained by the far-red nuclear stain RedDot2, and TUNEL staining (green). Arrows highlight TUNEL+ nuclei. Middle and right , immunostaining of the PFC and DStr for the apoptosis marker cleaved caspase 3 (CC3, green) and microglial marker Iba1 (red), counterstained with nuclear marker RedDot2 (blue). Arrows denote co-localization of all three signals. ( D ) Quantification of TUNEL+ cells in the PFC and DStr of male and female rats self-administering METH (n = 5–7 per group), MDPV (n = 6–8 per group), or saline (n = 4–9 per group). * p < 0.05 vs. corresponding saline groups. ( E ) Quantification of co-localized CC3, Iba1, and nuclei in the PFC and DStr of male and female rats self-administering METH (n = 4–6 per group), MDPV (n = 4–6 per group), or saline (n = 5–6 per group). * p < 0.05 vs. corresponding saline groups. All data are shown as mean ± SEM. Open circles represent data values from individual animals.

    Journal: Brain Sciences

    Article Title: Methamphetamine and the Synthetic Cathinone 3,4-Methylenedioxypyrovalerone (MDPV) Produce Persistent Effects on Prefrontal and Striatal Microglial Morphology and Neuroimmune Signaling Following Repeated Binge-like Intake in Male and Female Rats

    doi: 10.3390/brainsci14050435

    Figure Lengend Snippet: ( A ) Tiled confocal images of a representative coronal hemisphere stained for Iba1. ( B ) Compared to saline controls (n = 5–9 per group), decreased densities of Iba1+ cells were observed in the PFC of male and female animals self-administering METH (n = 5–7 per group). * p < 0.05 vs. saline. ( C ) Left , representative tissue section showing outlines of nuclei (red) stained by the far-red nuclear stain RedDot2, and TUNEL staining (green). Arrows highlight TUNEL+ nuclei. Middle and right , immunostaining of the PFC and DStr for the apoptosis marker cleaved caspase 3 (CC3, green) and microglial marker Iba1 (red), counterstained with nuclear marker RedDot2 (blue). Arrows denote co-localization of all three signals. ( D ) Quantification of TUNEL+ cells in the PFC and DStr of male and female rats self-administering METH (n = 5–7 per group), MDPV (n = 6–8 per group), or saline (n = 4–9 per group). * p < 0.05 vs. corresponding saline groups. ( E ) Quantification of co-localized CC3, Iba1, and nuclei in the PFC and DStr of male and female rats self-administering METH (n = 4–6 per group), MDPV (n = 4–6 per group), or saline (n = 5–6 per group). * p < 0.05 vs. corresponding saline groups. All data are shown as mean ± SEM. Open circles represent data values from individual animals.

    Article Snippet: Sections were then incubated with a 1× solution of the far-red fluorescent nuclear stain RedDot2 (Biotium, Fremont, CA, USA) for 30 min; washed 1 × 10 min in PBS; coverslipped; sealed; and stored at 4 °C, protected from light, until analysis.

    Techniques: Staining, Saline, TUNEL Assay, Immunostaining, Marker